[pp.45-52]

Experimental Study of Cloning from Stem Segment in Seventeen Poaceae Species

Taizo UCHIDA, Teruo ARASE and Fuyuki TAZAKI （Kyushu Sangyo University / Shinshu University / Hokkaisuiko Consultant Inc.）

Abstract:
As an aid to weed risk assessment, presence of cloning from stem segments of the seventeen Poaceae species causing trouble as weedy or invasive plants in Japan was checked under laboratory conditions. Stems of the above species were cut into several segments, 20 cm length with one node, and then soaked in the distilled water at 25 °C with 12 h of lights for 40 days. As the results showed, among the seventeen Poaceae species tested, cloning from their stem segments was observed in the eight species (47.1 % of all). Also, all of the clones once formed had adventitious roots. These results would indicate that stem segments of some Poaceae species became a medium for their propagation and/or dispersal like rhizome and seed. Therefore, it would be useful as a measure of the weed risk assessments to check the cloning from stem segments of Poaceae species. On the other hand, investigating the effects of temperature (5 °C, 10 °C, 15 °C, 20 °C, 25 °C and 30 °C) and stem segment length (5 cm, 15 cm and 25 cm) on the cloning from stem segments, it was shown that the cloning from stem segments of some Poaceae species would be dependent on temperature, even though segment length class would not affect the cloning.

Key Word:
clone, Poaceae, stem segment, weed risk assessment

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